LC/MS

Vedanta-Our-ServicesVedanta lab believes in prioritizing the customer above all. Hence, our services are solely focused on flawless accuracy, effective timeliness and exemplary customer service. Our specifically designed clinical tools are shaped accordingly so that we can help you treat your patient at the right time, with the right medication.

Technology has come a long way for the toxicology labs, which is why we do not hesitate to innovate and remain in step with the evolving health care challenges. We do so through the employment of the most recent and effective technological services.

One such leading example is our use of liquid chromatography-mass spectrometry (LC/MS) that has come to be known as a routine technique based on the development of electrospray ionization. LC/MS forms the basis for a simple and robust interface that can be applied to a wide range of biological molecules.

The use of MS and stable isotope internal standards allows the development of highly sensitive and accurate assays.

LC/MS ensures faster scanning speeds that widen the scope for a greater degree of multiplexing and the measurement of many compounds in a single analytical run. The development of more affordable and reliable instruments has complemented LC/MS to start playing an important role in several areas of clinical biochemistry and compete with conventional techniques such as immunoassay.

Unlike gas chromatography, which is not suitable for non-volatile and thermally fragile molecules, liquid chromatography can safely separate a wide range of organic compounds, from small-molecule drug metabolites to peptides and proteins.

Mass spectrometers also generate three dimensional data. In addition to signal strength, they generate mass spectral data that can provide valuable information about the molecular weight, structure, identity, quantity and purity of a sample. Mass spectral data adds specificity to the results of both qualitative and quantitative analyses.

The sensitive and highly specific nature of MS makes it more desirable to be combined with chromatographic techniques than other chromatographic detectors. For most compounds, a mass spectrometer is more sensitive and far more specific than all other LC detectors. Mass spectral data complements data from other LC detectors. It can also identify components in unresolved chromatographic peaks, eradicating the need for perfect chromatography. Together they offer high accuracy and increased ability to handle complex mixtures.

LC/MS is quickly growing into the gold standard for clinical intelligence and assessments.

LC/MS is suitable for many applications, stretching across pharmaceutical development to environmental analysis and biochemical screening for genetic disorders.

It offers wide applications for therapeutic drug monitoring and toxicology due to its greater specificity regarding identifications, detection of a wider range of drugs, recognition of toxins and their metabolites and simplification of sample preparation. Its applications extend to measurements of vitamins and related metabolites and steroid hormones.

Other fundamental applications of LC/MS include the determination of molecular weights (key to determining identity) and the determination of information about molecular structure.

We still make use of immunoassays for the initial screening test in order to be efficient for time-sensitive cases. However, we emphasize the confirmation of all initial results through a more detailed and thorough analysis that LC/MS provides.

According to the Substance Abuse and Mental Health Services Administration (SAMHSA), the other benefits of drug testing in primary care include:

  • Screening, assessing and diagnosing substance abuse or addiction
  • Preventing dangerous interactions of pharmaceuticals during surgery or other procedures
  • Verifying, contradicting or adding to self-reports or interviews detailing a patient’s substance use behavior
  • Overseeing abstinence progress and detecting cases of relapse

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